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Literature summary extracted from

  • Qin, N.; Peitzsch, M.; Menschikowski, M.; Siegert, G.; Pacak, K.; Eisenhofer, G.
    Double stable isotope ultra performance liquid chromatographic-tandem mass spectrometric quantification of tissue content and activity of phenylethanolamine N-methyltransferase, the crucial enzyme responsible for synthesis of epinephrine (2013), Anal. Bioanal. Chem., 405, 1713-1719.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.1.28 analysis method to measure tissue contents and activity of phenylethanolamine N-methyltransferase, utilizing double stable isotope ultra performance liquid chromatography-tandem mass spectrometry. The method is based on measurement of deuterium-labeled epinephrine produced from reaction of norepinephrine with deuterium-labeled S-adenosyl-Lmethionine as the methyl donor. In addition to enzyme activity the method allows for determination of tissue contents of PNMT using human recombinant enzyme for calibration. The calibration curve for epinephrine is linear over the range of 0.1 to 5000 pM, with 0.5 pM epinephrine representing the lower limit of quantification Homo sapiens
2.1.1.28 medicine strong relationship between PNMT gene expression and tissue epinephrine levels in human pheochromocytomas Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.28 Homo sapiens
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.28 additional information tissue from pheochromocytoma tumor specimens Homo sapiens
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Synonyms

EC Number Synonyms Comment Organism
2.1.1.28 PNMT
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 Homo sapiens

Expression

EC Number Organism Comment Expression
2.1.1.28 Homo sapiens strong relationship between PNMT gene expression and tissue epinephrine levels in human pheochromocytomas additional information